Cellular engineering

Avin Babataheri - Abdul Barakat - Julien Husson


Sawicka et al. Molecular Biology of the Cell 2017(in press).
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Hogan et al., Biophysical Journal 2015. 109 (2): 209–219 - pre-print
Gonzalez-Rodriguez et al. PRL 115, 088102 - pre-print

How to quantify cell mechanical properties and their evolution over time ?

We study cellular and intracellular mechanics, specifically in leukocytes, endothelial cells, and leukocyte-endothelium interactions. We are interested in the study of how mechanical aspects are involved in cellular physiological and pathological processes. For instance, we study leukocyte transendothelial migration in the concext of the development of atherosclerosis, T lymphocyte activation and the formation of the immunological synapse, cytotoxic function of T lymphocytes, as well as phagocytosis. We are also interested in the mechanics of cell membrane.

In order to perform these studies, we develop and use single-cell micromanipulation techniques based on micropipettes. These allow us to measure forces generated by cells, the viscoelastic properties of the latter, and their evolution over time.

Micropipette Force Probe and T Lymphocyte
Profile microindentation of an endothelial cell