Diagnostique cellulaire


How to produce concentration gradients in microfluidics?

Cyprien Guermonprez - Sébatien Michelin - Charles Baroud

Why ?

Concentration gradients are important in microfluidics for biomechanicals and screening assays. For example, bacteria response to an antibiotic depends on the agent concentration.

Methods

Flow rates distribution within channels.


Advection - Diffusion


Results

Flow rate distribution with branches
Branch concentration profile is Péclet dependant

Applications

Array of micro-reactors with diverse and controlled concentrations


Can we control thousands of parallel cell colonies in square centimeter ?

R. Tomasi - G. Amselem - S. Sart C. N. Baroud

Why?

Single-cell studies allow to investigate cellular heterogeneity in large populations, which is crucial in many fields such as cancer therapy ot stemcell isolation.

How?

Microdroplets can be efficiently trapped by anchors etched in the channel ceiling.


We built a high density array on a microfluidic chip

564 droplets trapped on anchors

Results

Droplet production
3D cell culture in collagen

Publications

Publication : R. Dangla, S. Lee and C. Baroud. Trapping microfluidic drops in wells of surface energy. Physical Review Letters, 107:124-501. Phys. Rev. Lett. 107, 124501. preprint